王皓毅，博士，研究員，必威精装版app西汉姆联 幹細胞與生殖生物學國家重點實驗室副主任，基因工程技術研究組組長。2009年於美國華盛頓大學獲得分子細胞生物學博士學位，開發了基於轉座子的Calling Card技術。2009年至2014年在麻省理工學院Whitehead研究所Rudolf Jaenisch實驗室進行博士後研究，從事全能性幹細胞和小鼠中基因編輯技術的開發和應用。2014年加入必威精装版app西汉姆联 ，擔任基因工程技術研究組組長。在近年研究中取得多項成果，包括在人類多能性幹細胞和CAR-T細胞中建立了高效的基因編輯方法；建立基因編輯以及胚胎電轉構建小鼠模型方法；建立人類naive胚胎幹細胞培養條件，並基於此實現人類X染色體隨機失活的模擬；建立原位基因表達調控技術CRISPR-on和Casillio係統等。相關研究成果發表在Cell、Nature Biotechnology、Cell Stem Cell、Genome Research、Cell Research等雜誌上，已發表SCI學術論文五十餘篇，其中（共同）第一作者和通訊作者文章37篇，總引用次數已過萬次 (google scholar統計)。回國後已獲得國家自然科學基金優秀青年基金、麵上項目、中科院幹細胞先導專項以及科技部幹細胞與再生醫學重點研發計劃等經費資助。

　　基因工程技術的開發和應用

　　近期研究方向：

　　1. 開發源頭創新的基因工程技術。

　　2. 在T細胞和幹細胞中建立和優化基因編輯技術，開發新型疾病治療方法。

　　3. 利用人類胚胎幹細胞技術研究早期胚胎發育中的X染色體失活現象的分子機製。

（*共同第一作者, #通訊作者）

1. Li N*, Tang N, Cheng C, Hu T, Wei X, Han W, Wang H#. “Improving the anti-solid tumor efficacy of CAR-T cells by inhibiting adenosine signaling pathway.” OncoImmunology, 2020 Sep 24 Online.
2. Sun W*, Wang H#. (2020). “Recent advances of genome editing and related technologies in China”. Gene Therapy, 2020 Aug; 27(7-8): 312-320. 
3. An C*, Feng G*, Zhang J, Cao S, Wang Y, Wang N, Lu F, Zhou Q, Wang H#. “Overcoming Autocrine FGF Signaling-Induced Heterogeneity in Naive Human ESCs Enables Modeling of Random X Chromosome Inactivation.” Cell Stem Cell, 2020 Sep; 27（3）: 482-497.e4. 
4. Tang N*, Cheng C*, Zhang X, Qiao M, Li N, Mu W, Wei X, Han W, Wang H#. “TGF β Inhibition via CRISPR Promotes the Long-Term Efficacy of CAR-T Cells Against Solid Tumors.” JCI Insight, 2020 Feb; 5(4): e133977. 
5. Cao Y*, Sun W*, Wang J, Sheng G, Xiang G, Zhang T, Shi W, Li C, Wang Y, Zhao F#, Wang H#. “Argonaute proteins from human gastrointestinal bacteria catalyze DNA-guided cleavage of single- and double- stranded DNA at 37 °C.” Cell Discovery, 2019; 5:38.
6. Wang H#，Yang H#. “Gene-edited babies: What went wrong and what could go wrong.” PLOS Biology, 2019 Apr 30 Online.
7. Mu W*, Tang N*, Cheng C*, Sun W, Wei X, Wang H#. “In vitro transcribed sgRNA causes cell death by inducing interferon release.” Protein & Cell, 2019 Jan 7 Online. 
8. Cheng C*, Tang N*, Li J, Cao S, Zhang T, Wei X, Wang H#. “ Bacteria-free minicircle DNA system to generate integration-free CAR-T cells.” Journal of Medical Genetics, 2019 Jan; 56(1): 10-17. 
9. Xiang G*, Ren J*, Hai T*, Fu R, Yu D, Wang J, Li W, Wang H#, Zhou Q#. “Editing porcine IGF2 regulatory element improved meat production in Chinese Bama pigs.”  Cellular and Molecular Life Sciences. 2018 Dec; 75(24): 4619-4628.  
10. Xiang G, Wang H#. “Extended pluripotent stem cells facilitate mouse model generation.”  Protein & Cell. 2018 Aug 21. doi: 1007/s13238-018-0573-0.
11. Mu W, Zhang Y, Xue X, Liu L, Wei X, Wang H#.” 5′ capped and 3′ polyA-tailed sgRNAs enhance the efficiency of CRISPR-Cas9 system.” Protein & Cell, 2018. June Online. 
12. Zhang Y, Zhang X, Cheng C, Mu W, Liu X, Li N, Wei X, Liu X, Xia C, Wang H^#.“CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells.” Frontiers of Medicine, 2017 Dec;11(4):554-562.
13. Xiang G, Zhang X, An C, Cheng C, Wang H^#.“Temperature effect on CRISPR-Cas9 mediated genome editing.”Journal of Genetics and Genomics, 2017 Apr;44(4):199-205.
14. Liu X, Zhang Y, Cheng C, Cheng WA, Zhang X, Li N, Xia C, Wei X, Liu X, Wang H^#. “CRISPR-Cas9 mediated multiplex gene editing in CAR-T cells.” Cell Research, 2017 Jan;27(1):154-157.
15. Burgess S, Cheng L^#, Gu F, Huang J, Huang Z, Lin S^#, Li J, Li W, Qin W, Sun W, Songyang Z, Wei W^#, Wu Q, Wang H^#, Wang X, Xiong JW, Xi J, Yang H, Zhou B, Zhang B. “Questions about NgAgo” Protein Cell, 2016 Dec;7(12):913-915.
16. Wang W,Kutny PM, Byers SL, Longstaff CJ, DaCosta MJ, Pang C, Zhang Y, Taft RA, Buaas FW, Wang H^#. “Delivery of Cas9 protein into mouse zygotes through a series of electroporation dramatically increased the efficiency of model creation.” Journal of Genetics and Genomics, 2016 May 20;43(5):319-27.
17. Qin W, Kutny PM, Maser RS, Dion SL, Lamont JD, Zhang Y, Perry GA, Wang H^#.“Generating Mouse Models Using CRISPR-Cas9 Mediated Genome Editing.” Current Protocols in Mouse Biology, 2016 Mar 1;6(1):39-66. 
18. Cheng AW*^#, Jillette N*, Lee P, Plaskon D, Fujiwara Y, Wang W, Taghbalout A, Wang H^#. “Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling.” Cell Research, 2016 Feb;26(2):254-7. 
19. Wiles MV, Qin W, Cheng AW, Wang H^#. “CRISPR-Cas9 mediated genome editing and guide RNA design.” Mammalian Genome, 2015 Oct;26(9-10):501-10.
20. Qin W, Dion SL, Kutny PM, Zhang Y, Cheng AW, Jillette NL, Malhotra A, Geurts AM, Chen YG, Wang H^#. “Efficient CRISPR/Cas9-mediated genome editing in mice by zygote electroporation of nuclease.” Genetics, 2015 Jun;200(2):423-30.
21. Theunissen T*, Powell B*, Wang H*, Mitalipova M, Faddah D, Reddy J, Fan Z, Maetzel D, Ganz K, Shi L, Lungjangwa T, Imsoonthornruksa S, Stelzer Y, Rangarajan S, D’Alessio A, Zhang J, Gao Q, Dawlaty M, Young R, Gray N, Jaenisch R. “Systematic Identification of Culture Conditions for Induction and Maintenance of Naive Human Pluripotency.”Cell Stem Cell, 2014 Oct 2; 15(4):471-87.
22. Yang H, Wang H, Jaenisch R. “Generating genetically modified mice using CRISPR/Cas-mediated genome engineering.” Nature protocols, 2014 Aug; 9(8):1956-68.
23. Maetzel D*,Sarkar S*, Wang H*, Mosleh LA, Cheng AW, Xu P, Gao Q, Mitalipova M, Jaenisch R. “Genetic and chemical correction of cholesterol accumulation and impaired autophagy in hepatic and neural cells derived from Niemann-Pick Type C patient-specific iPS cells.” Stem Cell Reports, 2014 May 15; 2(6):866-80.
24. Cheng AW*, Wang H*, Yang H, Shi L, Katz Y, Rangarajan S, Theunissen TW, Shivalila CS, Dadon DB, Jaenisch R. “Multiplexed activation of endogenous genes by CRISPR-on, a RNA-guided transcriptional activator system.” Cell Research, 2013 October 1; 23(10): 1163-1171.
25. Yang H*, Wang H*, Shivalila CS*, Cheng AW, Shi L, Jaenisch R. “One-step generation of mice carrying reporter and conditional allele by CRISPR/Cas mediated genome editing” Cell, 2013 September 12; 154(6):1370-1379.
26. Faddah D, Wang H, Buganim Y, Cheng AW, Jaenisch R. “Expression of Nanog is biallelic and equally variable as other pluripotency factors.” Cell Stem Cell, 2013 Jul 3;13(1):23-9.
27. Wang H*, Hu YC*, Markoulaki S, Welstead GG, Shivalila CS, Cheng AW, Pyntikova T, Dadon D, Voytas DF, Bogdanove AJ, Page DC, Jaenisch R. “TALEN-mediated editing of the Mouse Y Chromosome.” Nature Biotechnology, 2013 Jun; 31(6):530-2.
28. Wang H*, Yang H*, Shivalila CS*, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R. “One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas mediated genome engineering.” Cell, 2013 May 9;153(4):910-8. 

2真正好的科學研究是充滿樂趣和成就感的，希望我們能一起體驗科學的魅力。

2024年招生視頻：https://mp.weixin.qq.com/s/ehTuLIQEq5v9r1SioC5TsQ